Reagents

Lipase from porcine pancreas (Sigma-Aldrich, USA, Cat.№ L3126)

Tris Base (Sigma-Aldrich, USA, Cat.№ 252859)

Trimethylsulfonium Iodide, 98% (Acros Organics, India, Cat.№ 140250250)

Silver(I) oxide, 99+% (Acros Organics, UK, Cat.№ 205800100)

2',7'-Dichlorofluorescein, pure (Acros Organics, Germany, Cat.№ 191530050)

2,6-Di-tert-butyl-4-methylphenol, 99.8% (Acros Organics, Belgium, Cat.№ 219830500)

Trifluoroacetic anhydride (TFAH), 98% (Macklin, China, Cat.№ T818925)

2-Amino-2-methyl-1-propanol (AMP) (Macklin, China, Cat.№ A800065)

Acetic Acid glacial (USP, BP, Ph. Eur., JP) pure, pharma grade (Panreac, Germany, Cat.№ 141008)

Desoxycholic acid sodium salt (Sigma-Aldrich, Germany, Cat.№ D6750)

ACETONE 99.8% For HPLC & UV Spectroscopy (Loba Chemie, India, Cat.№ 00013)

Diethyl ether (Honeywell, Germany, Cat.№ 32203)

2-Propanol (Sigma-Aldrich, France, Cat.№ 34863)

Acetonitrile RS - For HPLC PLUS Gradient grade - ACS - Reag.Ph.Eur. - Reag.USP (Carlo Erba, France, Cat.№ 412392000)

n-Hexane, 99+%, for analysis (Acros Organics, Belgium, Cat.№ AC160780025)

Methanol, HPLC Grade, 99.8+% (Fisher Chemical, Trinidad and Tobago, Cat.№ 11318157)

Silica gel on TLC Al foils (Fluka Analytical, Germany, Cat.№ 02599)

TLC aluminum sheets, Silica gel 60 (Supelco, Germany, Cat.№ 1055530001)

TLC aluminum sheets, Silica gel 60 (Merck, Germany, Cat.№ 1168350001)