Анализ \(FA\) в \(TL\) семян *Acer*s с помощью реакции

  • Клен серебристый (Acer saccharinum)

  • Клен красный (Acer rubrum)

  • Клен монпелийский (Acer monspessulanum)

  • Клен семенова (Acer semenovii)

  • Клен татарский (Acer tataricum)

  • Клен полевой (Acer campestre)

  • Клен каппадокийский (Acer cappadocicum)

  • Клен моно (Acer mono)

  • Клен завитой (Acer circinatum)

  • Клен ложнозибольдов (Acer pseudosieboldianum)

  • Клен пенсильванский (Acer pensylvanicum)

  • Клен зеленокорый (Acer tegmentosum)

  • Клен колосистый (Acer spicatum)

  • Клен желтый (Acer ukurunduense)

  • Клен сахарный (Acer saccharum) (~октябрь)

  • Клен маньчжурский (Acer mandshuricum)

  • Клен ясенелистный (Acer negundo)

  • Клен псевдоплатанус (Acer pseudoplatanus) (~октябрь)

  • Клен приречный (Acer ginnala)

Метод

  1. Растереть пробу семян пестиком в пробирке

  2. Внести \(MeOH\): 500 \(\mu L\)

  3. Внести \(NaOCH_3\)/\(H_2O\) (1%): 100 \(\mu L\)

  4. Вортексировать

  5. Греть 55 \(^\circ C\), 30 \(min\)

  6. Внести \(H_2O\): 250 \(\mu L\)

  7. Внести \(Heptane\): 300 \(\mu L\)

  8. Вортексировать

  9. Центрифугировать 3 \(min\), 2300 \(g\)

  10. Отобрать верхнюю фазу (\(Heptane\))

Изображения

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