Helianthus Annuus

# Identifier
1 К-2233, Прогресс, Russia
2 К-2699, Прогресс, Russia
3 К-3599, RIL-130, France
4 К-3675, ВИР 839, Russia
5 К-3384, ВИР 584, Russia
6 К-3714, ВИР 172Б, Russia
7 К-2776, ВИР 136, Russia

Note

Sample 3 was not peeled.

# Mass of test tube, \(g\) Mass of test tube with sample, \(g\) Mass of sample, \(g\) Number of seeds
1 33.6330 36.3481 2.7151 50
2 33.6319 36.8111 3.1792 50
3 33.5919 36.0650 2.4731 75
4 33.5914 35.7894 2.1980 75
5 33.4945 35.8355 2.3410 75
6 33.5618 35.6694 2.1076 50
7 33.5006 36.4158 2.9152 50
  1. Add \(CHCl_3/MeOH\) (1/2, \(v/v\)): 12 \(mL\).
    • Shake: 10 min.
  2. Add \(CHCl_3\): 4 \(mL\).
    • Shake: 5 min.
  3. Add \(H_2O\): 4 \(mL\).
    • Shake: 5 min.
    • Centrifuge: 5 \(min\), 2300 \(g\).
  4. Collect bottom phase.
  5. Substract top phase.

  6. Repeate 1-6.

  7. Add \(Na_2SO_4\): 1 ложку (для вытягивания воды).
    • Shake: 5 min.

Filter and Evaporate

Tip

The collected sample must be heated to room temperature.

  1. Filter.
  2. Evaporate.
  3. Add \(Acetone\) (it mixes with water and dissolves oil).
    • Evaporate.
    • Weigh (wait until the mass stops changing).
  4. Add \(C_6H_6\) (~ 200 \(mg/mL\)).
# Mass of test tube, \(g\) Mass of test tube with extract, \(g\) Mass of extract, \(g\) \(C_6H_6\), \(mL\)
1 26.7727 28.0419 1.2692 6.3460
2 36.6262 38.0810 1.4548 7.2740
3 26.7727 27.7014 0.9287 4.6435
4 36.6262 37.6500 1.0238 5.1190
5 26.7727 28.0850 1.3123 6.5615
6 36.6262 37.5928 0.9666 4.8330
7 26.7727 28.0290 1.2563 6.2815
# Extract mass to sample mass, %
1 46.75
2 45.76
3 37.55
4 46.58
5 56.06
6 45.86
7 43.09

TLC

  1. Add: 100 \(mg\) (0.5 \(mL\)).
    • Подвижная фаза \(Hexane/Et_2O\) (75/25, \(v/v\)).

  2. Опрыскать $Primuline$

  3. Add: \(Hexane/Et_2O\) (1/1, \(v/v\)).
    • Centrifuge: 5 \(min\), 2300 \(g\).
  4. Перенести в чистые виалы.
    • Упарить.
    • Взвесить.
  5. Add \(C_6H_6\) (~ 100 \(mg/mL\)).
# Mass of test tube, \(g\) Mass of test tube with extract, \(g\) Mass of extract, \(g\) \(C_6H_6\), \(mL\)
1 2.4981 2.5535 0.0554 0.554
2 2.4239 2.4883 0.0644 0.644
3 2.4454 2.5227 0.0773 0.773
4 2.4295 2.5085 0.0790 0.790
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